Crm1 and p53
WebJul 3, 2013 · Chromosomal region maintenance 1 (CRM1) mediates p53 nuclear export. Although p53 SUMOylation promotes its nuclear export, the underlying mechanism is unclear. Here we show that tethering of a … WebJun 28, 2024 · Overexpression of CRM1 in cervical cancer cell lines reduced the nuclear retention of several tumor suppressors including p53, p27, p21, and p18. siRNA-induced inhibition of CRM1 in cervical cancer …
Crm1 and p53
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WebMay 16, 2013 · p53 is shuttled between the nucleus and the cytoplasm. 1,15 Exportin 1 (chromosomal region maintenance 1 [CRM1]) is a member of nuclear export receptors … WebJul 3, 2013 · The transfected cells were fixed 24 h after transfection and stained with antibodies to p53 and Nup153. p53, CRM1, and Nup153 were detected in the blue, green and red channels, respectively. Colocalization of CRM1, p53-SUMO-1, and Nup153 at the NPCs, as well as that of CRM1 and p53-SUMO-1 in the cytoplasm, is denoted with white …
WebMay 1, 2015 · p53 Phosphorylation in Lung Tumors from NNK-Treated Mice and in NNK-Transformed Lung Epithelial Cells. CRM1 plays an important role in the transport of tumor suppressor and oncogene proteins, including p53, from the cell nucleus into the cytoplasm. 35, 36 Our previous study found p53 phosphorylation at Thr55 involved in CRM1 … WebMar 15, 2024 · Using proximity ligation assay (PLA) on NBAT1 sh and Csh SH-SY5Y cells, we observed that p53 and CRM1 interaction was higher in the NBAT1 sh cells when …
WebJul 3, 2013 · The CRM1 C-terminal domain lacking the NES-binding groove interacts with tetrameric p53, and the proper folding of the p53 core domain, rather than the presence of the N or C-terminal tails,... WebFeb 1, 2024 · Blood Cancer Journal - XPO1 inhibitors represent a novel therapeutic option in Adult T-cell Leukemia, triggering p53-mediated caspase-dependent apoptosis. ... (CRM1/XPO1), the key nuclear ...
WebCRM1, p53, and topo IIα protein expression and intracellular localization As shown by immunofluorescence microscopy, baseline CRM1 (green) nuclear localization and overall expression were high in untreated human MM cells and human MM cells treated with the inactive SINE trans-isomer KPT185T (Fig. 2 A, first column).
WebHere, we identify the major poly (ADP-ribosyl)ated sites of p53 by PARP-1 and find that PARP-1-mediated poly (ADP-ribosyl)ation blocks the interaction between p53 and the … toughcheckWebDec 1, 2012 · These results further substantiated p53 involvement in CRM1 inhibition–mediated apoptosis and cell-cycle arrest exclusively in the wt p53 cells. Mdm2-mediated p53 ubiquitinylation exposes the latter's NES, promoting its interaction with CRM1 with a consequent enhancement of its nuclear export and cytoplasmic degradation … pottery barn dallas ft worthWebApr 4, 2009 · Crm1 is known to export p53 from the nucleus into the cytoplasm of cervical cancer cells via the C-terminal NES of p53, 28 a function that is promoted by HPV E6. 21 Additionally, nuclear export is required for the efficient degradation of p53 in high-risk HPV-infected cells, 28 although nuclear degradation of p53 does occur at low levels. 21 ... tough cfexpressWebCRM1 and Ran-GDP then diffuse back to the nucleus where GEFs exchange GDP to GTP . Because nuclear localization of proteins such as transcription factors is key to their function, and an adequate localization of signaling proteins close to their downstream targets can be imperative for many signal transduction pathways, unbalanced ... pottery barn dallas hoursWebMay 16, 2013 · High CRM1 expression was associated with short survival of patients and remained an adverse prognostic factor in multivariate analysis. CRM1 inhibitor KPT-185 induced mainly full-length p53 and apoptosis in a p53-dependent manner, whereas inhibition of proliferation was p53 independent. tough challenge synonymsWebCRM1 inhibition rescued p53-dependent nuclear functions and sensitized NBAT1-depleted cells to genotoxic drugs. Combined inhibition of CRM1 and MDM2 was even more … tough cfo interview questionsWebInhibition of CRM1-mediated nuclear export by Leptomycin B resulted in nuclear accumulation of COMMD1. In addition, low oxygen concentrations induced the active export of COMMD1 from the nucleus in a CRM1-dependent manner. Disruption of the NESs in COMMD1 increased the repression of COMMD1 in transcriptional activity of NF-kappa B … pottery barn dallas galleria