Sphere formation cells
WebNational Center for Biotechnology Information WebThe subpopulation of sphere-forming cells performs most stem cells’ properties, which is instrumental for stem cell studies. In HeLa cells, the sphere-forming cells express various stem cell markers in an extremely high amount, including OCT4 and SOX2.
Sphere formation cells
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WebJun 14, 2011 · To evaluate the stemness characteristics of spheres, the self-renewal, proliferation, chemoresistance, tumorigenicity of the PLC/PRF/5 sphere-forming cells, and … WebFeb 1, 2024 · A, The cell proliferation and viability (OD 490) were measured in Flo-1 and Flo-1 XTR, the radiation-resistant cells at days 3 and 6 using MTS assay, respectively, as described in Materials and Methods. B, Representative images (top) and quantification (low) of tumor sphere formation in Flo-1 and Flo-1 XTR cells are demonstrated.
WebThe effect of IL-8 on stemness of sphere-forming cells was determined through tumor sphere formation, wound healing migration, and in vivo tumorigenesis assays. Results: In our study, uPAR + and uPAR - cells showed different gene expression profiles. IL-8 was upregulated in SCLC sphere-forming cells. WebFeb 13, 2024 · The harvested spheroids were also dissociated into single cells by incubating the spheroids in enzymatic solution using 0.25% trypsin-EDTA (Gibco USA) for 30 min followed by mechanical dissociation using pipetting. The harvested single cells suspension were subjected to the following assays.
WebNov 1, 2024 · Whereafter, we carried out sphere formation, colony formation, and EdU assays to evaluate the effect of NF-Exo and CAF-Exo on RCC cells. As shown in Fig. 3D–F, CAF-Exo significantly... WebJun 4, 2013 · Sphere formation was observed in 13/26 cell lines. Comparison to molecular phenotypic data from other studies , , , revealed no clear correlation with sphere formation . Non-sphere-forming and sphere-forming cells lines fell in similar proportions amongst luminal-like and basal-like categories , . Collation of data on cell line in ...
WebJul 17, 2015 · BORIS silencing studies showed a decrease of sphere formation capacity in breast and colon tumor cells. Importantly, BORIS-silencing led to down-regulation of …
WebThe sphere-formation efficiency of a culture (number of spheres divided by the number of seeded cells x 100) is a proxy for the self-renewal ability of that culture. The described … asian paint luster paintWebDec 21, 2012 · For sphere assays, 500 cells were seeded per well in 96-well plates and transduced with nontargeting or STAT3 shRNA lentiviral particles, and the number of spheres was counted after 14 days. For western blots, ... neurosphere formation, and stem cell marker expression in vitro. The results presented here thus validate the potency of … atacan a rusiaWebInterestingly, 100 ng/mL doxycycline already significantly limited sphere formation in PANC-1 cells but had no effect in MIA PaCa-2 cells. The 2000 ng/mL doxycycline dose again drastically suppressed sphere formation by PANC-1 and also, to some extent, reduced the number of spheres formed by MIA PaCa-2 cells although this difference did not ... atacan kievWebJan 23, 2024 · Sphere formation from a single sorted cancer cell was induced by hypoxia As floating sphere-like proliferation is a basic feature of CSCs, 19 it was necessary to investigate whether hypoxia... asian paint mail loginWebNeurospheres each contain cells at multiple stages of differentiation, including stem cells, proliferating neural progenitor cells, postmitotic neurons, and glia. Moreover, the … asian paint msds pdfWebThe clones with more than 50 cells were collected and cells were counted. In a particular setting of colony formation, sphere culture-enriched cells were adjusted to a density of … atacan koleji yorumlarWebDec 23, 2024 · The treatment of miR-526b-3p mimic suppressed the sphere formation counts of breast cancer cells and inhibited ALDH1 and Nanog expression. MiR-526b-3p was able to target HIF-2α in the cells. The overexpression enhanced but miR-526b-3p reduced the IC 50 value of PTX in breast cancer cells, in which the overexpression of HIF-2α could … atacan koleji