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Top/fop flash原理

WebWe next investigated the effect of irradiation on Wnt/β-catenin pathway activity using TOP/FOP flash luciferase assays and quantitative polymerase chain reaction analysis of β-catenin target genes. The expression levels and activities of two target genes, matrix metalloproteinase (MMP)-2 and MMP-9, were examined further by Western blotting ... Web用western和免疫荧光验证beta－catenin和下游靶基因的变化只是基础，只是说明你转染了这个抑癌基因的确是改变了catenin通路，但是你想要证明该抑癌基因是通过catenin通路来 …

生物学研究中，什么是TOPflash和FOPflash? - 搜狗问问

Web26. dec 2024 · 原理： 经典的Wnt信号通路需要beta-catenin入核，进而与转录因子TCF/LEF结合形成复合物，共同起始下游调控基因的转录。 据此，科学家通过将数个拷贝 … WebTo measure Wnt signaling, one can use a variety of Wnt reporters. The TOP-flash assay ( Molenaar 1996) is widely used and variants of TOP-Flash (SuperTop) are available from … long use of prednisone side effects https://smediamoo.com

HK2通过Wnt/β-catenin信号通路上调Cyclin D1、MMP7和Slug表达 …

Web25. máj 2024 · 载体描述. The Wnt/Wg family encodes secreted glycoproteins implicated in cell growth regulation, cell fate determination, organogenesis and oncogenesis. Inferred from genetic analysis of wingless (wg) signaling in Drosophila, in mammalian cells, Wnts signal through the Frizzled family of receptors, to Dishevelled. WebSupplementary Information Materials and Methods TOPFlash/FOPFlash assays. TOPFlash is a luciferase reporter that contains a minimal fos promoter coupled to Tcf-binding sites … WebTour Start here for a quick overview of the site Help Center Detailed answers to any questions you might have Meta Discuss the workings and policies of this site long u shaped kitchen

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Category:molecular biology - How does a TOPflash/FOPflash assay work to detect

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Top/fop flash原理

molecular biology - How does a TOPflash/FOPflash assay work to detect

WebTOP or FOP plasmid + Opti-MEM and Lipofectamine + Opti-MEM are incubated at room temp for 5 min respectively. Both solutions are then mixed and incubated for another 15 … WebF , The expression of β-catenin by nuclear/cytoplasmic protein fractionation and TOP/FOP-flash reporter assays when silencing MBD2a or overexpressing MBD2c. Data were from 3 independent experiments. ∗ P .05; ∗∗ P .01 by the Student t test. G , The expression of Snail1, OCT4, SOX2 mRNA, and proteins was measured by qPCR and Western blot ...

Top/fop flash原理

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WebTOPFlash(报告基因质粒)是用于检测Wnt信号通路中β-catenin介导的TCF/LEF转录活性水平的报告基因质粒。 TOPFlash是以碧云天的pGL6-TA为模板，在其多克隆位点插入两 … WebFOPFlash(报告基因质粒)是TOPFlash(D2501)的阴性对照报告基因质粒。

Web図3 TOP-Flash/FOP-Flashルシフェ ラーゼアッセイシステム 図4 Calotropis giganteaより単離した 化合物 化学と生物 Vol. 52, No. 11, 2014735 活性のみを低下させていたことか …

Web21. feb 2012 · TOPflash is a TCF reporter plasmid containing two sets of three copies of wild-type TCF binding sites driven by the thymidine kinase minimal prompter and … WebArticle Snippet: A total of 50 ng TOP/FOP-Flash vectors (Promega Corporation) and 5 pmol miR-NC inhibitor or miR-934 inhibitor were co-transfected into 5,000 HCT116 cells using Lipofectamine 3000 (Invitrogen; Thermo Fisher Scientific, Inc.) at 37˚C for 48 h. The TOP/FOP ratio was measured to assess the activity of Wnt/β-catenin signaling via ...

Web2016-08-30 Adobe Flash Player 22 Activex ... 14 2016-02-05 英文“flash”翻译成中文是什么意思？ 6

WebTOPflash (TCF Reporter Plasmid) Transfection grade T-cell factor reporter plasmid containing 2 sets (with the second set in the reverse orientation) of 3 copies of the TCF … longus mean in latinWebTOP/FOP-FLASH assay in HEK293T cells? Question. 7 answers. Asked 29th Aug, 2024; Yen Teng Tai; Has anyone tried to study Wnt signaling in HEK293T cells? I have been trying to measure the Wnt ... long u short uWebPrice from $9.99 to $1999.99. top fop flash assay - by Bioz Stars , 2024-12. 90 / 100 stars. Buy from Supplier. top fop flash vector ( Promega ) 99. Promega top fop flash vector. Top Fop Flash Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. hopkins sporting clays mdWeb10. jan 2024 · For the TOP/FOP-Flash reporter assay, the TOP/FOP-Flash reporter and pTK-RL plasmids were co-transfected into cells. After transfection for 48 h, the cells were harvested for analysis with the ... long u short storyWeb通常以TOP/FOP的比值表示。 公式如下： 转染有TOP-Flash和海肾荧光素载体的样本中采集得到的萤火虫荧光值F(Top)/采集得到的海肾荧光值R(Top) 转染有FOP-Flash和海肾荧光 … hopkins stages theatreWeb( http://www.abnova.com ) - Firefly luciferase is widely used as a reporter to study gene expression and other cellular events. The Luciferase Assay is an ex... long u short u worksheetWeb一般而言，topflash/fopflash是比较常用的检测β-catenin的系统，原理和所有的RLU一样，都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基因的表达。 如 … long u short u worksheets